A permanent position for an INRA research scientist is available at the DynAMic laboratory of Vandœuvre-lès-Nancy. The research topic is on biochemistry of the conjugation machinery of Gram+ bacteria.
Applications are open until 2018 mars 5, at 17h on http://jobs.inra.fr/offers/emploi_perm
Contact : Pr Nathalie Leblond-Bourget, head of the ICE-TeA team.
E-mail : email@example.com. Phone : (+33) 3 72 74 51 46
The DynAMic laboratory is a research unit headed by INRA and Lorraine University. The laboratory is interested in the evolution of bacterial genomes, in gene transfers and their impact on bacterial adaptation. The successful candidate will join one of the two teams of the unit: the ICE-TeA team (ICE-Transfer and Adaptation), composed of 1 professor, 5 assistant professors, 1 INRA senior researcher, 3 technicians, and non-permanent staff (1 post-doc, 1 PhD and 1 technician at the moment).
ICEs (Integrative and Conjugative Elements) are mobile genetic elements integrated in bacterial genomes, that can excise from the chromosome and transfer by conjugation. They often carry genes conferring an adaptive potential for the host cell, as antibiotic resistance or virulence genes. ICEs are autonomous for their transfer, encoding a conjugation pore belonging to type IV secretion systems (T4SS).
The candidate will join a research axis of the team aiming at the understanding of the molecular and structural mechanisms allowing the recruitment of the DNA to be transferred and the assembly of the conjugation pore of firmicutes (Gram+), belonging to the genus Streptococcus.
The candidate will possess a robust experience in protein biochemistry. He-she will characterize the conjugation machinery of ICEs, deciphering structure-function relationships of the multiprotein complex that defines the conjugation pore. He-she will develop strategies to control gene transfers, in collaboration with specialists in structural biology and molecular modelling. As the conjugation is a transmembrane complex, an expertise in membrane protein purification would be a plus, as basis in 3D structure determination.
The DynAMic unit is located within the scientific technopole Henri Poincaré, a prosperous environment allowing multidisciplinary interactions. Several platforms are available, including the ones of the scientific federation EFABA (INRA- Lorraine University) and the biophysics platform of the Biopôle (CNRS-Lorraine University). The DynAMic unit has also developed a scientific network of local collaborators including cristallographs, chemists and computer scientists, aiming at the understanding of gene transfers performed by ICEs, and at the inhibition of these transfers. We also collaborate internationally with Elisabeth Grohmann’s laboratory (working on Gram+ conjugative plasmids), and with Gabriel Waksman’s laboratory (expert of cryoEM).